α brca (Novus Biologicals)
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Novus Biologicals
α brca
α Brca, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/α brca/product/Novus Biologicals
Average 94 stars, based on 5 article reviews
α Brca, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/α brca/product/Novus Biologicals
Average 94 stars, based on 5 article reviews
α brca - by Bioz Stars,
2026-05
94/100 stars
Images
1) Product Images from "Gasdermin C sensitizes tumor cells to PARP inhibitor therapy in cancer models"
Article Title: Gasdermin C sensitizes tumor cells to PARP inhibitor therapy in cancer models
Journal: The Journal of Clinical Investigation
doi: 10.1172/JCI166841
Figure Legend Snippet: ( A ) MDA-MB-436 stable cells as indicated were inoculated into the mammary fat pad of nude mice ( n = 10). Mice were administered olaparib. Tumor growth was shown. ( B and C ) 4TO7 cells harboring an empty vector (vector) or expressing WT mouse Gsdmc ( Gsdmc -WT) or the caspase-8 cleavage site D263A mutant ( Gsdmc -mut) were inoculated into the mammary fat pad of nude mice ( B ) or immunocompetent BALB/c mice ( C ) ( n = 10). Mice were administered olaparib. Tumor growth was shown. ( D ) LDH level in tumor slurry of tumors indicated in C was measured. ( E and F ) Same as B and C , except that stable transfectants were established in 4TO7- Brca KO instead of 4TO7 parental cells and injected ( n = 10). ( G ) LDH level in slurry of tumors indicated in F was measured. ( H ) Parental 4TO7 cells mixed with 0%, 15%, or 30% 4TO7- Brca –KO Gsdmc -WT cells were inoculated into BALB/c mice ( n = 10). Mice were administered olaparib. Tumor growth was shown. ( I ) BALB/c mice with 4TO7- Brca –KO Gsdmc -WT or vector tumors were administered olaparib and durable tumor regression was monitored ( n = 10). ( J and K ) 4TO7- Gsdmc -WT ( J ) or 4TO7- Brca –KO Gsdmc -WT ( K ) cells were inoculated into the mammary fat pad of nude mice and immunocompetent BALB/c mice ( n = 10). Mice were administered olaparib. Survival curves were shown. Data represent mean ± SD. 1-way ANOVA was used for A – H . The log-rank test was used for J and K . *** P < 0.001.
Techniques Used: Plasmid Preparation, Expressing, Mutagenesis, Injection
Figure Legend Snippet: ( A ) 4TO7- Brca –KO cells stably expressing an empty vector (vector) or WT mouse Gsdmc ( Gsdmc -WT) or the D263A mutant ( Gsdmc -mut) were inoculated into the mammary fat pad of immunocompetent BALB/c mice ( n = 10). Mice were administered olaparib. Percentage of tumor-infiltrating CD8 + T cells was analyzed. ( B ) Overexpression of eGFP in the stable cells as indicated in A . Then cells and mice were treated same as in A . Mean numbers of eGFP tetramer + (eGFP tet + ) CD8 + T cells per gram of tumor (left). Percentage of IFN-γ + (middle) or TNF-α + (right) CD8 + T cells activated by eGFP peptide. ( C ) Frequency of memory T cell subsets in lymph node (LN), spleen, and tumors of A. Tex, exhausted T cell. ( D ) Initial tumor challenge and mice treatment were same as in A . Tumors were removed on day 18. Then tumor rechallenge of 4TO7 parental cells was performed 60 days after tumor removal. Tumor growth was shown ( n = 10). ( E ) Stable cells as indicated in A were inoculated into the mammary fat pad of immunocompetent BALB/c mice ( n = 10). 4TO7 parental cells were simultaneously injected into contralateral mammary fat pad. Mice were administered olaparib. Tumor growth of 4TO7 parental cells was monitored. ( F and G ) 4TO7- Brca –KO Gsdmc -WT cells were inoculated into BALB/c mice ( n = 10). Mice were administered olaparib. Depletion of CD8 + T cell with anti-CD8. Curves of tumor growth ( F ) and survival ( G ). ( H ) Growth curve of 4TO7- Gsdmc -WT and 4TO7-vector tumors in BALB/c mice ( n = 10) treated with olaparib or PD-1 antibody or the combination. Data represent mean ± SD. 1-way ANOVA was used for A , B , D , E , and H . Unpaired 2-tailed t test was used for F . Log-rank test was used for G . ** P < 0.01, *** P < 0.001.
Techniques Used: Stable Transfection, Expressing, Plasmid Preparation, Mutagenesis, Over Expression, Injection
Figure Legend Snippet: ( A – F ) MDA-MB-157 and Hs578t cells with deletion of BRCA . Cells were treated with the indicated concentrations of olaparib for 72 hours and subjected to a cell viability assay ( n = 3) ( A and B ). Immunoblotting of GSDMC cleavage in cells treated with the indicated concentrations of olaparib for 72 hours ( C and D ). Cell death measured by LDH release (LDH-released cell death) induced by olaparib at the indicated concentrations ( n = 3) ( E and F ). ( G and H ) 4TO7 parental or Brca -KO cells with ectopic expression of Gsdmc were inoculated into the mammary fat pad of immunocompetent BALB/c mice ( n = 10). Mice were administered olaparib (50 mg/kg) 5 times per week for 18 days. Tumor growth ( G ) and survival ( H ) curves were shown. ( I ) Ectopic expression of Gsdmc in parental or Brca -KO cells of PanO2, MC38, Hepa-1-6, B16. Cells were inoculated into the mammary fat pad of immunocompetent C57BL/6 mice ( n = 10). Mice were treated same as G . Tumor growth curves were shown. Data represent mean ± SD. Unpaired 2-tailed t test was used for A and B . 2-way ANOVA was used for E and F . 1-way ANOVA was used for G and I . Log-rank test was used for H . *** P < 0.001.
Techniques Used: Viability Assay, Western Blot, Expressing